What question did this study set out to answer?

To evaluate the capability of three Claviceps purpurea strains isolated from rye sclerotia to synthesize specific peptide ergot alkaloids in submerged culture.

January 1, 1969Applied MicrobiologyOpen Access

Production of Peptide Ergot Alkaloids in Submerged Culture by Three Isolates of Claviceps purpurea

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To evaluate the capability of three Claviceps purpurea strains isolated from rye sclerotia to synthesize specific peptide ergot alkaloids in submerged culture.
Cultivated three Claviceps purpurea strains isolated from rye sclerotia under submerged fermentation conditions.
Monitored conidia formation, growth dynamics, nutrient uptake (phosphate, ammonium nitrogen, and citric acid), and accumulation of lipids, sterols, and alkaloids.
Each strain synthesized distinct alkaloid profiles under submerged conditions: ergocryptine with ergotamine, ergocornine with ergosine, and ergocristine alone, respectively.
All strains formed conidia sparingly or not at all, but accumulated high levels of intracellular lipids and sterols.
Culture progression followed two distinct phases: an initial phase of rapid nutrient uptake and biomass growth with low alkaloid production, followed by a decelerated growth phase characterized by marked accumulation of lipids, sterols, and alkaloids.

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Implication

In vitro fermentation study reveals biphasic alkaloid synthesis in Claviceps purpurea isolates, indicating that nutrient depletion triggers secondary metabolite production.

Cite This Study

Amici et al. (1969) studied this question.

synapsesocial.com/papers/6a717a12ce524a4339c4ff1c https://doi.org/10.1128/aem.18.3.464-468.1969

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Implication

In vitro fermentation study reveals biphasic alkaloid synthesis in Claviceps purpurea isolates, indicating that nutrient depletion triggers secondary metabolite production.

Production of Peptide Ergot Alkaloids in Submerged Culture by Three Isolates of Claviceps purpurea

Key Points

Discussion

Implication

Cite This Study

Production of Peptide Ergot Alkaloids in Submerged Culture by Three Isolates of Claviceps purpurea

Key Points

Discussion

Implication

Cite This Study

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