What question did this study set out to answer?

To identify the physiological and nutritional characteristics associated with high ergotamine and peptide alkaloid production in Claviceps purpurea grown in submerged culture.

May 1, 1967Applied MicrobiologyOpen Access

Ergotamine Production in Submerged Culture and Physiology of Claviceps purpurea

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Authors

AAA. AmiciUniversità degli Studi della Tuscia AMA. Minghetti TST. ScottiRutgers, The State University of New Jersey

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Implication

Comparative in vitro study reveals enhanced alkaloid yield in Claviceps purpurea strains, highlighting the necessity of simultaneous sucrose and citric acid metabolism.

Key Points

To identify the physiological and nutritional characteristics associated with high ergotamine and peptide alkaloid production in Claviceps purpurea grown in submerged culture.
Compared the high-producing Claviceps purpurea strain 275 FI against three spontaneous mutant strains (V, C, and W) unable to synthesize substantial alkaloids.
Assessed cellular accumulation of lipids and sterols across all strains during submerged culture growth.
Tracked and compared the consumption kinetics of sucrose and citric acid substrates in the culture media.
The high-producing strain 275 FI accumulated substantial intracellular lipids and sterols, whereas non-producing mutant strains showed an absence of this accumulation capacity.
High alkaloid yield occurred strictly when cells utilized large amounts of sucrose and citric acid simultaneously, unlike mutant strains that consumed only sucrose (strain V), only citric acid (strain C), or minimal amounts of both (strain W).

Cite This Study

Amici et al. (1967) studied this question.

synapsesocial.com/papers/6a717a12ce524a4339c4ff1d https://doi.org/10.1128/am.15.3.597-602.1967

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Implication

Comparative in vitro study reveals enhanced alkaloid yield in Claviceps purpurea strains, highlighting the necessity of simultaneous sucrose and citric acid metabolism.

Ergotamine Production in Submerged Culture and Physiology of Claviceps purpurea

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Implication

Key Points

Cite This Study

Ergotamine Production in Submerged Culture and Physiology of Claviceps purpurea

Discussion

Member takes

Implication

Key Points

Cite This Study

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