What question did this study set out to answer?

To determine the cellular and vascular mechanisms through which recombinant human erythropoietin modulates vascular tone and induces hypertension.

April 1, 1992Journal of Cardiovascular Pharmacology

Endothelin Release and Shift in Prostaglandin Balance Are Involved in the Modulation of Vascular Tone by Recombinant Erythropoietin

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To determine the cellular and vascular mechanisms through which recombinant human erythropoietin modulates vascular tone and induces hypertension.
Assessed vascular contractions and relaxations in isolated rabbit aortic rings and human renal artery rings exposed to rhEPO in combination with norepinephrine, acetylcholine, indomethacin, or aspirin.
Quantified the release of prostanoids (PGI2, PGF2 alpha, PGE2, TxB2) and endothelin-1 via radioimmunoassay in cultured human umbilical vein endothelial cells treated with 200 U/ml rhEPO.
rhEPO showed no direct contractile activity and did not impair acetylcholine-induced nitric oxide relaxation, but it potentiated norepinephrine-induced contractions in rabbit aorta.
Indomethacin and aspirin abolished rhEPO-induced increases in constrictor prostanoids in aortic and renal artery rings, yet only partially reduced the enhancement of norepinephrine contractions.
In cultured human umbilical vein endothelial cells, rhEPO (200 U/ml) increased PGF2 alpha and TxB2, reduced PGI2, and increased endothelin-1 secretion by nearly 90%.

Structured PICO

Population

Rabbit aorta rings, human renal artery rings, and cultured human umbilical vein endothelial cells (HUVEC)

Intervention

Recombinant human erythropoietin (rhEPO) (e.g., 200 U/ml)

Comparator

Control (absence of rhEPO)

Outcome

Vascular tone (norepinephrine-induced contractions) and synthesis of prostanoids and endothelin-1surrogate

rhEPO may cause hypertension by shifting the endothelial prostanoid balance toward vasoconstriction and increasing endothelin-1 release.

Discussion

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Implication

rhEPO shifts vascular prostanoid balance toward constriction and boosts endothelin-1 in animals; leaves open its contribution to clinical hypertension.

Cite This Study

Bode‐Böger et al. (1992) studied this question.

synapsesocial.com/papers/6a718744ce524a4339c504cf https://doi.org/10.1097/00005344-199204002-00009

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Implication

rhEPO shifts vascular prostanoid balance toward constriction and boosts endothelin-1 in animals; leaves open its contribution to clinical hypertension.

Endothelin Release and Shift in Prostaglandin Balance Are Involved in the Modulation of Vascular Tone by Recombinant Erythropoietin

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Endothelin Release and Shift in Prostaglandin Balance Are Involved in the Modulation of Vascular Tone by Recombinant Erythropoietin

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider