What question did this study set out to answer?

To determine whether recombinant human erythropoietin administration increases vascular nitric oxide production, endothelial nitric oxide synthase expression, and nitric oxide-dependent vasodilation in healthy rats.

September 26, 2003Journal of Cardiovascular Pharmacology

Erythropoietin Administration In Vivo Increases Vascular Nitric Oxide Synthase Expression

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Why the study?

Does recombinant human erythropoietin administration increase vascular nitric oxide production and endothelial expression of nitric oxide synthase in healthy rats?

Population

Healthy male rats instrumented with pulsed Doppler flow probes around their ascending aorta and with…

Comparison

Recombinant human erythropoietin 2 U/d for 14 days vs Vehicle for 14 days

Design

Preclinical

Follow-up

14 days

Authors

NKNancy L. KanagyUniversity of New Mexico MPMichael F. PerrineUniversity of New Mexico DCDora K. Cheung

Discussion

Member takes

Implication

rHuEpo raises MAP while upregulating vascular NOS in rats; leaves open human relevance and clinical translation.

Key Points

To determine whether recombinant human erythropoietin administration increases vascular nitric oxide production, endothelial nitric oxide synthase expression, and nitric oxide-dependent vasodilation in healthy rats.
Healthy male rats were instrumented with ascending aorta pulsed Doppler flow probes alongside arterial and femoral catheters.
Animals received daily treatment with recombinant human erythropoietin (2 U/day) or vehicle for 14 days.
Vascular reactivity of phenylephrine-contracted thoracic aorta to acetylcholine and a nitric oxide donor was evaluated, alongside hemodynamic responses to nitric oxide synthase inhibition via N-omega-nitro-L-arginine (10 and 30 mg/kg).
Erythropoietin treatment elevated hematocrit and significantly raised mean arterial pressure compared to vehicle controls (142 ± 3 vs. 116 ± 4 mm Hg).
Thoracic aorta segments from treated rats showed a modest increase in nitric oxide-dependent relaxation to acetylcholine, while relaxation to the donor SNAP and contractions to phenylephrine did not differ from controls.
Nitric oxide synthase inhibition caused significantly greater increases in blood pressure and total peripheral resistance in treated rats, accompanied by increased aortic NOS expression and plasma NOx levels.

Structured PICO

Does recombinant human erythropoietin administration increase vascular nitric oxide production and endothelial expression of nitric oxide synthase in healthy rats?

Population

Healthy male rats instrumented with pulsed Doppler flow probes around their ascending aorta and with arterial and femoral catheters

Intervention

Recombinant human erythropoietin (rHuEpo) 2 U/d for 14 days

Comparator

Vehicle for 14 days

Outcome

Vascular nitric oxide (NO) production, endothelial expression of nitric oxide synthase, and NO-dependent vasodilationsurrogate

In vivo administration of recombinant human erythropoietin increases vascular eNOS expression, which appears to cause basal vasodilation in rHuEpo-induced hypertensive rats.

Cite This Study

Kanagy et al. (2003) studied this question.

synapsesocial.com/papers/6a849a6e8aee47fac4e26d3c https://doi.org/10.1097/00005344-200310000-00011

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Erythropoietin Administration In Vivo Increases Vascular Nitric Oxide Synthase Expression

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Erythropoietin Administration In Vivo Increases Vascular Nitric Oxide Synthase Expression

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider