September 1, 1992AJP Heart and Circulatory Physiology

Na+ efflux mechanisms in ventricular myocytes: measurement of Na+i with Na(+)-binding benzofuran isophthalate

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Population

Isolated, adult rat ventricular myocytes

Design

Preclinical

Authors

SBS. BorzakBrigham and Women's Hospital MRMartin ReersBehringwerke (Germany)JAJose A. ArrudaHospital Universitário Evangélico de Curitiba

Discussion

Member takes

Implication

Offers no immediate clinical guidance; hypothesis-generating for Na+ pump dominance in isolated myocyte efflux.

Structured PICO

Population

Isolated, adult rat ventricular myocytes

Intervention

Loading with Na(+)-sensitive dye benzofuran isophthalate (SBFI) via incubation with 10 microM of the acetoxymethyl ester; experimental manipulation of extracellular ions (substitution of Ca2+ and Mg2+ with EGTA) and pharmacological blockade (1 microM verapamil, inhibition of Na(+)-K(+)-ATPase)

Outcome

Intracellular Na+ concentration ([Na+]i) and characterization of Na+ efflux mechanismssurrogate

SBFI can be used to nondestructively measure intracellular Na+ in ventricular myocytes, demonstrating that Na+ efflux under specific loading conditions is driven by the Na+ pump rather than Na+-Ca2+ exchange.

Cite This Study

Borzak et al. (1992) studied this question.

synapsesocial.com/papers/6a718ce82163a0a01bc5a4a3 https://doi.org/10.1152/ajpheart.1992.263.3.h866

View Full Paper

Ask AI

Bookmark

Na+ efflux mechanisms in ventricular myocytes: measurement of Na+i with Na(+)-binding benzofuran isophthalate

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Na+ efflux mechanisms in ventricular myocytes: measurement of Na+i with Na(+)-binding benzofuran isophthalate

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider