RGS2 deficiency in mice resulted in a strong hypertensive phenotype, increasing mean arterial pressure by 51 mmHg compared to wild-type controls.
Mean Difference: 51
Absolute Event Rate: 135% vs 84%
p-value: p=<0.0005
Signaling by hormones and neurotransmitters that activate G protein-coupled receptors (GPCRs) maintains blood pressure within the normal range despite large changes in cardiac output that can occur within seconds. This implies that blood pressure regulation requires precise kinetic control of GPCR signaling. To test this hypothesis, we analyzed mice deficient in RGS2, a GTPase-activating protein that greatly accelerates the deactivation rate of heterotrimeric G proteins in vitro. Both rgs2+/- and rgs2-/- mice exhibited a strong hypertensive phenotype, renovascular abnormalities, persistent constriction of the resistance vasculature, and prolonged response of the vasculature to vasoconstrictors in vivo. Analysis of P2Y receptor-mediated Ca2+ signaling in vascular smooth muscle cells in vitro indicated that loss of RGS2 increased agonist potency and efficacy and slowed the kinetics of signal termination. These results establish that abnormally prolonged signaling by G protein-coupled vasoconstrictor receptors can contribute to the onset of hypertension, and they suggest that genetic defects affecting the function or expression of RGS2 may be novel risk factors for development of hypertension in humans.
Heximer et al. (Sat,) conducted a other in Hypertension. RGS2 deficiency (rgs2-/-) vs. Wild-type mice was evaluated on Mean arterial pressure (MAP) (MD 51 mmHg, p=<0.0005). RGS2 deficiency in mice resulted in a strong hypertensive phenotype, increasing mean arterial pressure by 51 mmHg compared to wild-type controls.