What question did this study set out to answer?

To determine whether cocaine stimulates the release of the vasoconstrictor endothelin-1 and to identify the underlying endothelial receptor mechanisms.

August 4, 1998CirculationOpen Access

Cocaine Increases the Endothelial Release of Immunoreactive Endothelin and Its Concentrations in Human Plasma and Urine

View Full Paper

Ask AI

Bookmark

Authors

UWUte Wilbert-LampenGerman Centre for Cardiovascular Research CSChristian SeligerMerck (Germany)TZThomas ZilkerTechnical University of Munich

Discussion

Member takes

Implication

Translational study demonstrates elevated endothelin-1 levels in cocaine-intoxicated patients and endothelial cells, indicating that sigma-receptor stimulation contributes to cocaine-induced...

Key Points

To determine whether cocaine stimulates the release of the vasoconstrictor endothelin-1 and to identify the underlying endothelial receptor mechanisms.
Measured endothelin-1 via radioimmunoassay and HPLC in porcine aortic endothelial cell culture supernatants exposed to cocaine (10⁻⁷ to 10⁻⁴ mol/L) and sigma-receptor antagonists haloperidol (10⁻⁶ mol/L) or ditolylguanidine (10⁻⁵ mol/L).
Characterized endothelial membrane binding sites using [3H]ditolylguanidine (25×10⁻⁹ mol/L) radioligand assays.
Quantified plasma and urine endothelin-1 concentrations in 12 cocaine-intoxicated patients and 13 healthy control subjects.
In endothelial cell cultures, cocaine dose-dependently increased endothelin-1 release rates to a plateau of 175±23% of control at hours 4 to 5, an effect abolished or reduced by haloperidol and ditolylguanidine.
Endothelial membranes exhibited specific binding for [3H]ditolylguanidine, with an estimated 1,400 sigma-receptor binding sites per cell.
Cocaine-intoxicated patients had significantly higher endothelin-1 levels than controls in plasma (22.7±5.6 vs 7.3±0.8 pmol/L) and urine (41.5±10.1 vs 12.7±3.8 pmol/L).

Cite This Study

Wilbert-Lampen et al. (1998) studied this question.

synapsesocial.com/papers/6a71b65ff44fa9f079df7103 https://doi.org/10.1161/01.cir.98.5.385

View Full Paper

Ask AI

Bookmark

Implication

Translational study demonstrates elevated endothelin-1 levels in cocaine-intoxicated patients and endothelial cells, indicating that sigma-receptor stimulation contributes to cocaine-induced...

Cocaine Increases the Endothelial Release of Immunoreactive Endothelin and Its Concentrations in Human Plasma and Urine

Discussion

Member takes

Implication

Key Points

Cite This Study

Cocaine Increases the Endothelial Release of Immunoreactive Endothelin and Its Concentrations in Human Plasma and Urine

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider