December 1, 1995Journal of VirologyOpen Access

The 3' untranslated region of picornavirus RNA: features required for efficient genome replication

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Population

Mutants derived from either poliovirus type 3 or a PV3 replicon containing the reporter gene chloramphenicol…

Comparison

Replacement of PV3 3'UTR with heterologous… vs Wild-type PV3 or PV3 replicon

Design

Preclinical

Authors

JRJonathan B. RohllUniversity College of Osteopathy DMDavid H. MoonDuke University DEDavid J. EvansUniversity of Oxford

Discussion

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Implication

May guide 3'UTR-targeted antivirals in picornaviruses; leaves open validation in human cardiac models.

Structured PICO

Population

Mutants derived from either poliovirus type 3 (PV3) or a PV3 replicon containing the reporter gene chloramphenicol acetyltransferase

Intervention

Replacement of PV3 3'UTR with heterologous 3'UTRs (coxsackie B4, HRV14, bovine enterovirus, hepatitis A virus), site-directed mutagenesis of HRV14 3'UTR, and insertion of a 1.1-kb heterologous sequence

Comparator

Wild-type PV3 or PV3 replicon

Outcome

Viral genome replication levelssurrogate

The 3'UTR of picornaviruses requires specific structural features, including stem stability and proximity to the poly(A) tail, for efficient genome replication.

Cite This Study

Rohll et al. (1995) studied this question.

synapsesocial.com/papers/6a7212e52163a0a01bc5ec7c https://doi.org/10.1128/jvi.69.12.7835-7844.1995

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The 3' untranslated region of picornavirus RNA: features required for efficient genome replication

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The 3' untranslated region of picornavirus RNA: features required for efficient genome replication

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider