February 1, 1995The Journal of General PhysiologyOpen Access

Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.

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Population

HEK293 cells transiently expressing the rabbit cardiac calcium channel alpha 1 subunit (alpha 1C)

Comparison

Coexpression of the rabbit calcium channel beta… vs Expression of the rabbit cardiac calcium channel…

Design

Preclinical

Authors

MPM. Teresa Pérez‐GarcíaUniversidad de Valladolid TKTimothy J. KampElectrophysiology EME MarbánJohns Hopkins University

Discussion

Member takes

Implication

May guide studies of L-type channel regulation; leaves open in vivo cardiac relevance and therapeutic potential.

Structured PICO

Population

HEK293 cells transiently expressing the rabbit cardiac calcium channel alpha 1 subunit (alpha 1C)

Intervention

Coexpression of the rabbit calcium channel beta subunit cloned from skeletal muscle

Comparator

Expression of the rabbit cardiac calcium channel alpha 1 subunit (alpha 1C) alone

Outcome

Current density, number of high-affinity dihydropyridine binding sites, and macroscopic kinetics of the current (activation and inactivation rates)surrogate

Coexpression of the beta subunit with the cardiac alpha 1C calcium channel subunit in HEK293 cells modulates channel gating and increases the number of functional channels.

Cite This Study

Pérez‐García et al. (1995) studied this question.

synapsesocial.com/papers/6a725e5b84cc45bb7be01c35 https://doi.org/10.1085/jgp.105.2.289

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Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider