May 1, 1987Journal of Biological ChemistryOpen Access

The hydrolytic cycle of sarcoplasmic reticulum Ca2+-ATPase in the absence of calcium.

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Population

Sarcoplasmic reticulum Ca2+-ATPase preparation

Comparison

Dimethyl sulfoxide (up to 15% and 30%) vs Absence of dimethyl sulfoxide or optimal…

Design

Preclinical

Authors

PCPaulo C. Carvalho-AlvesUniversidade Federal do Rio de Janeiro HSHelena M. ScofanoInstituto Biológico

Discussion

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Implication

DMSO may enable Ca2+-independent SERCA activity in vitro; hypothesis-generating for cardiac regulation, no practice change.

Structured PICO

Population

Sarcoplasmic reticulum Ca2+-ATPase preparation

Intervention

Dimethyl sulfoxide (up to 15% and 30%)

Comparator

Absence of dimethyl sulfoxide or optimal conditions (pH 7.5, 10 microM CaCl2, 100 mM KCl at 37 degrees C)

Outcome

Velocity of ATP hydrolysis and enzyme phosphorylationsurrogate

Dimethyl sulfoxide can bypass Ca2+ activation to stimulate Mg2+-dependent ATP hydrolysis of sarcoplasmic reticulum Ca2+-ATPase in the absence of calcium.

Cite This Study

Carvalho-Alves et al. (1987) studied this question.

synapsesocial.com/papers/6a7264fe75498292b70b811a https://doi.org/10.1016/s0021-9258(18)48285-3

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The hydrolytic cycle of sarcoplasmic reticulum Ca2+-ATPase in the absence of calcium.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The hydrolytic cycle of sarcoplasmic reticulum Ca2+-ATPase in the absence of calcium.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

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