Key result
C12E8 solubilization of sarcoplasmic reticulum renders phosphoenzyme decomposition Mg2+-independent until detergent removal.
Why the study?
The effect of solubilization with a high concentration of C12E8 detergent on the Mg2+ sensitivity of the phosphoenzyme in sarcoplasmic reticulum Ca2+-ATPase was unclear.
Population
Sarcoplasmic reticulum membranes from rabbit skeletal muscle
Comparison
Solubilization with high concentration C12E8 vs removal of C12E8 by Bio-Beads
Design
Preclinical biochemical study
Authors
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Reversible C12E8 effects on SERCA kinetics; leaves open relevance to cardiac Ca2+ handling in vivo.
Solubilization of sarcoplasmic reticulum Ca2+-ATPase with C12E8 causes reversible desensitization of phosphoenzyme decomposition to Mg2+.
Yamamoto et al. (1982) studied this question. C12E8 (dodecyl octaethyleneglycol monoether) vs. Removal of C12E8 was evaluated on Kinetic properties of Ca2+,Mg2+-dependent ATPase. Solubilization of sarcoplasmic reticulum with C12E8 renders phosphoenzyme decomposition Mg2+-independent, a property that is fully restored upon detergent removal.
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