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Experimental study demonstrates quantitative estimation of creatine and diacetyl using an alkaline color reaction, indicating viable analytical methods for biochemical assays.
Voges & Proskauer [1898] observed that when strong alkali was added to broth cultures of certain species of bacteria there developed, after an interval of time, a pink colour. Subsequently the work of Harden [1906] and of Harden & Norris [1911] established that the chemical reaction responsible was one between diacetyl and creatine (or certain similar substances). Attempts to make the reaction quantitative The reaction was further studied by Duliere [1929 The present paper presents some additional facts about the reaction and methods of estimation based on it.
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Eggleton et al. (1943) studied this question.
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