December 12, 2005Veterinary ResearchOpen Access

Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

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Why the study?

Does automated rRT-PCR improve the detection of FMDV in milk compared to virus isolation in dairy cows?

Population

4 dairy cows (2 experimentally infected with FMDV, 2 in-contact cows held in the same pen)

Comparison

Automated real-time reverse transcription… vs Virus isolation (VI) in cell culture

Design

Preclinical

Follow-up

4 weeks

Authors

SRScott M. ReidThe Pirbright Institute SPSatya ParidaThe Pirbright Institute DKDonald P. KingMarine Mammal Center

Discussion

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Implication

rRT-PCR may improve FMDV detection in dairy milk; leaves open validation for field surveillance in cattle.

Structured PICO

Does automated rRT-PCR improve the detection of FMDV in milk compared to virus isolation in dairy cows?

Population

4 dairy cows (2 experimentally infected with FMDV, 2 in-contact cows held in the same pen)

Intervention

Automated real-time reverse transcription polymerase chain reaction (rRT-PCR) on whole, skim, cream and cellular debris components of milk

Comparator

Virus isolation (VI) in cell culture

Outcome

Detection of FMDV in milk and milk fractionssurrogate

Automated rRT-PCR is quicker and more sensitive than virus isolation for detecting FMDV in whole milk and milk fractions from infected cattle.

Cite This Study

Reid et al. (2005) studied this question.

synapsesocial.com/papers/6a729dd2439bab0cabc3e2b9 https://doi.org/10.1051/vetres:2005040

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Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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