August 24, 1999Biochemistry

Detection of Nucleotide- and F-Actin-Induced Movements in the Switch II Helix of the Skeletal Myosin Using Its Differential Oxidative Cleavage Mediated by an Iron−EDTA Complex Disulfide-Linked to the Strong Actin Binding Site

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Population

Rabbit myosin subfragment-1 (S-1)

Design

Preclinical

Authors

RBRaoul BertrandCentre National de la Recherche Scientifique JCJean‐Paul CaponyCentre National de la Recherche Scientifique JDJean DerancourtCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

Supports proteolysis as a probe of myosin switch II transitions; hypothesis-generating for cardiac cross-bridge models and requires validation in higher systems.

Structured PICO

Population

Rabbit myosin subfragment-1 (S-1)

Intervention

S-(2-nitro-5-thiobenzoic acid) cysteaminyl-EDTA labeling and oxidative cleavage mediated by an iron-EDTA complex

Outcome

Yield of the 54 kDa-40 kDa pair of bands reflecting conformational changes in the switch II helixsurrogate

Actin site-specific chemical proteolysis of S-1 serves as a direct probe of switch II helix conformational transitions associated with the skeletal cross-bridge cycle.

Cite This Study

Bertrand et al. (1999) studied this question.

synapsesocial.com/papers/6a72c2d2f44fa9f079e02193 https://doi.org/10.1021/bi9909896

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Implication

Supports proteolysis as a probe of myosin switch II transitions; hypothesis-generating for cardiac cross-bridge models and requires validation in higher systems.

Detection of Nucleotide- and F-Actin-Induced Movements in the Switch II Helix of the Skeletal Myosin Using Its Differential Oxidative Cleavage Mediated by an Iron−EDTA Complex Disulfide-Linked to the Strong Actin Binding Site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Detection of Nucleotide- and F-Actin-Induced Movements in the Switch II Helix of the Skeletal Myosin Using Its Differential Oxidative Cleavage Mediated by an Iron−EDTA Complex Disulfide-Linked to the Strong Actin Binding Site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider