October 17, 2016The Journal of General PhysiologyOpen Access

Independent activation of ion conduction pores in the double-barreled calcium-activated chloride channel TMEM16A

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Population

TMEM16A calcium-activated chloride channel constructs

Comparison

Concatenated constructs containing subunits with… vs Wild-type (WT) subunits

Design

Preclinical

Authors

NLNovandy K. LimUniversity of Zurich ALAndy K.M. LamUniversity of Zurich RDRaimund DutzlerUniversity of Vienna

Discussion

Member takes

Implication

May inform targeted mutagenesis of TMEM16 Ca2+ sites; leaves open shared gating mechanism across scramblases and channels.

Structured PICO

Population

TMEM16A calcium-activated chloride channel constructs

Intervention

Concatenated constructs containing subunits with distinct activation and permeation properties, and mutation of a putative pore-lining residue

Comparator

Wild-type (WT) subunits

Outcome

Functional behavior including ion selectivity, conductance, and concentration and voltage dependence of channel activation by Ca2+surrogate

The TMEM16A calcium-activated chloride channel contains two independent ion conduction pores that are independently activated by intracellular calcium.

Cite This Study

Lim et al. (2016) studied this question.

synapsesocial.com/papers/6a72d4ba9da973292f08720e https://doi.org/10.1085/jgp.201611650

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Independent activation of ion conduction pores in the double-barreled calcium-activated chloride channel TMEM16A

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Independent activation of ion conduction pores in the double-barreled calcium-activated chloride channel TMEM16A

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider