Gi proteins and the response to 5‐hydroxytryptamine in porcine cultured endothelial cells with impaired release of EDRF

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Structured PICO

Population

Porcine cultured endothelial cells (model of regenerated endothelium) and native endothelial cells

Intervention

5-hydroxytryptamine (5-HT) stimulation (3 x 10-6 M; 4 min)

Comparator

Native endothelial cells and/or unstimulated cells

Outcome

Expression of Gi alpha proteins, ADP-ribosylation by pertussis toxin, and 5-HT binding sitessurrogate

The impaired release of EDRF in cultured porcine endothelial cells in response to 5-HT is not due to a loss of Gi alpha proteins or 5-HT receptors.

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Gi alpha loss does not explain EDRF dysfunction in culture; leaves open alternative mechanisms in endothelial signaling.

Day et al. (1995) studied this question.

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