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October 1, 1989Journal of Hypertension

Angiotensin formation in the isolated rat hindlimb

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Population

Isolated perfused rat hindquarters

Comparison

Perfusion with captopril, purified hog renin… vs Baseline spontaneous release or perfusion…

Design

Preclinical

Authors

KHKarl F. HilgersGeneral / Preventive / LipidsMKMarian KuczeraHeidelberg UniversityMWMarkus J. WilhelmUniversity Hospital of Zurich

Discussion

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Implication

Supports local vascular RAS in rat hindlimbs; hypothesis-generating for human hypertension therapies.

Key Points

  • To explore the local production of angiotensin and its effects in the isolated rat hindlimb.
  • Isolated perfusion of rat hindlimb with analysis using HPLC/radioimmunoassay
  • Assessment of angiotensin levels following interventions with captopril and hog renin
  • Evaluation of metabolic effects using synthetic tetradecapeptide and ACE inhibition.
  • Spontaneous release of Ang I (5.0 +/- 3.4 fmol/h) and Ang II (31.8 +/- 7.9 fmol/h) observed.
  • ACE inhibition with captopril abolished Ang II release; Ang I increased over 10-fold.
  • Approximately 50% of Ang I was converted to Ang II during one pass.

Structured PICO

P
Population
Isolated perfused rat hindquarters
I
Intervention
Perfusion with captopril (ACE inhibitor), purified hog renin, renin inhibitor H-142, synthetic tetradecapeptide renin substrate (TDP), Ang I, and Ang II
C
Comparator
Baseline spontaneous release or perfusion without inhibitors
O
Outcome
Angiotensin I and II levels in perfusate and vasoconstriction (perfusion pressure)surrogate

Demonstrates spontaneous release and local conversion of angiotensin in isolated rat hindlimbs, supporting the existence of a functional vascular renin-angiotensin system.

Cite This Study

Hilgers et al. (1989) studied this question.

synapsesocial.com/papers/6a72f929360ad7fbc59a2e8dhttps://doi.org/10.1097/00004872-198910000-00004
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Local generation and release of angiotensin II in peripheral vascular tissue.1988 · 93 citations
  2. 2Angiotensinogen is cleaved to angiotensin in isolated rat blood vessels.1993 · 21 citations
  3. 3Local Angiotensin II Generation in the Rat Heart1998 · 98 citations
  4. 4Vascular Angiotensin-Converting Enzyme Expression Regulates Local Angiotensin II1997 · 98 citations
  5. 5Local generation of angiotensin II as a mechanism of regulation of peripheral vascular tone in the rat.1984 · 127 citations