Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 1, 1993Hypertension

Angiotensinogen is cleaved to angiotensin in isolated rat blood vessels.

View Full Paper
Ask AI
Bookmark
Share

Key result

Addition of highly purified rat angiotensinogen to isolated rat hind limb perfusate increased angiotensin II release 14-fold compared with saline infusion.

Population

Bloodless, perfused, isolated hind limb preparation of the rat

Comparison

Perfusion with highly purified rat… vs Saline infusion or basal state

Design

Preclinical

Authors

KHKarl F. HilgersGeneral / Preventive / LipidsUHUlrich HilgenfeldtMax Planck SocietyRVRoland VeelkenFriedrich-Alexander-Universität Erlangen-Nürnberg

Discussion

Loading...

Member takes

Implication

Animal data support vascular renin uptake; leaves open its role in human hypertension and requires clinical validation.

Key Points

  • To investigate the conversion of angiotensinogen to angiotensin in isolated rat blood vessels.
  • Used a bloodless, perfused hind limb preparation of the rat
  • Measured angiotensinogen and angiotensin release using radioimmunoassay and high performance liquid chromatography
  • Administered captopril to assess its effect on angiotensin release
  • Spontaneous release of 4.7 +/- 1.5 pmol of angiotensinogen over 30 minutes
  • Captopril inhibited angiotensin II release by 67%, while increasing angiotensin I release by 301%
  • Bilateral nephrectomy reduced angiotensin release significantly by 95%

Structured PICO

P
Population
Isolated hind limb preparations from rats used to study the release of angiotensinogen and formation of angiotensins.
I
Intervention
Perfusion with highly purified rat angiotensinogen, captopril (10 mumol/L), or bilateral nephrectomy 24 hours prior
C
Comparator
Saline infusion or basal state
O
Outcome
Release of angiotensinogen and formation of angiotensins I and IIsurrogate

Main Result

Effect estimate: 14-fold increase

The study demonstrates that active renin is present in the vessel wall and can cleave natural angiotensinogen to form angiotensin peptides, supporting the concept that vascular renin is primarily taken up from the circulation.

Cite This Study

Hilgers et al. (1993) studied this question. Highly purified rat angiotensinogen vs. Saline infusion was evaluated on Release of angiotensin II (14-fold increase). Addition of highly purified rat angiotensinogen to isolated rat hind limb perfusate increased angiotensin II release 14-fold compared with saline infusion.

synapsesocial.com/papers/6a227e77ffccceb004b72ef9https://doi.org/10.1161/01.hyp.21.6.1030
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Angiotensin formation in the isolated rat hindlimb1989 · 68 citations
  2. 2Local generation and release of angiotensin II in peripheral vascular tissue.1988 · 93 citations
  3. 3Local generation of angiotensin II as a mechanism of regulation of peripheral vascular tone in the rat.1984 · 127 citations
  4. 4Angiotensinogen gene is expressed and differentially regulated in multiple tissues of the rat.1986 · 480 citations
  5. 5Expression of the renin gene in extra-renal tissues of the rat1988 · 99 citations