What question did this study set out to answer?

To establish a continuous intravenous radiotracer infusion method for quantifying the rate of brain protein synthesis and defining the precursor pool sources.

March 1, 1972Journal of Neurochemistry

A Technique for Measuring Brain Protein Synthesis

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Authors

PGP.J. GarlickUniversity of Bern IMIan C. B. MarshallHaitiChildren

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Implication

Preclinical study demonstrates intravenous radiolabeled tyrosine infusion measures brain protein synthesis rates in mice, indicating tissue proteins turn over with a four-day half-life.

Key Points

To establish a continuous intravenous radiotracer infusion method for quantifying the rate of brain protein synthesis and defining the precursor pool sources.
Infused mice intravenously with L-[U-14C]-tyrosine for durations ranging between 0.5 and 2 hours.
Measured the specific radioactivity of free tyrosine in both plasma and brain tissue, as well as protein-bound tyrosine in the brain, to calculate synthesis rates and precursor pool origin.
Calculated a mean half-life of approximately 4 days for mixed brain proteins during 0.5–2 hour infusions.
Identified that the intracellular precursor tyrosine pool is derived 60% directly from circulating plasma tyrosine and 40% from local tissue protein degradation.

Cite This Study

Garlick et al. (1972) studied this question.

synapsesocial.com/papers/6a7342dfd7374855d0c8f7d5 https://doi.org/10.1111/j.1471-4159.1972.tb01375.x

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Implication

Preclinical study demonstrates intravenous radiolabeled tyrosine infusion measures brain protein synthesis rates in mice, indicating tissue proteins turn over with a four-day half-life.

A Technique for Measuring Brain Protein Synthesis

Discussion

Member takes

Implication

Key Points

Cite This Study

A Technique for Measuring Brain Protein Synthesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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