October 1, 1997Lipids

Measurement of apolipoprotein B in various cell lines: Correlation between intracellular levels and rates of secretion

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Population

HepG2, Caco-2, and McA-RH7777 cell lines, including stably transfected McA-RH7777 cells

Design

Preclinical

Authors

ABAhmed BakillahKing Saud bin Abdulaziz University for Health Sciences ZZZhangyin Zhou JLJayraz LuchoomunKineMed (United States)

Discussion

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Implication

Cell model data warrant no practice change; leaves open lipidation-dependent apoB secretion as a therapeutic target.

Structured PICO

Population

HepG2, Caco-2, and McA-RH7777 cell lines, including stably transfected McA-RH7777 cells

Intervention

Standardization of enzyme-linked immunoassays using 0.5% taurocholate for solubilization

Outcome

Correlation between intracellular levels and rates of secretion of apolipoprotein B (apoB)surrogate

The rate of secretion of lipid-associated apoB, but not lipid-free apoB, is tightly controlled and correlates with intracellular levels in cell models.

Cite This Study

Bakillah et al. (1997) studied this question.

synapsesocial.com/papers/6a735d6664cdd4095c2c283d https://doi.org/10.1007/s11745-997-0143-8

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Measurement of apolipoprotein B in various cell lines: Correlation between intracellular levels and rates of secretion

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Measurement of apolipoprotein B in various cell lines: Correlation between intracellular levels and rates of secretion

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider