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December 1, 1995Journal of Biological ChemistryOpen Access

Gβγ Binds Directly to the G Protein-gated K+ Channel, IKACh

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Population

Immunoprecipitated cardiac IKACh and recombinant CIR and GIRK1 subunits

Comparison

Purified and recombinant beta and gamma subunits… vs G alpha (GTP gamma S- or GDP-bound)

Design

Preclinical

Authors

GKGrigory KrapivinskyBoston Children's HospitalLKLuba KrapivinskyBoston Children's HospitalKWKevin WickmanElectrophysiology

Discussion

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Implication

Provides molecular basis for Gβγ gating of cardiac IKACh; leaves open in vivo relevance and therapeutic targeting.

Structured PICO

P
Population
Immunoprecipitated cardiac IKACh and recombinant CIR and GIRK1 subunits
I
Intervention
Purified and recombinant beta and gamma subunits (G beta gamma) of heterotrimeric G proteins
C
Comparator
G alpha (GTP gamma S- or GDP-bound)
O
Outcome
Direct binding and dissociation constants (Kd) of G beta gamma to IKACh and its subunitssurrogate

G beta gamma binds directly and specifically to the cardiac IKACh channel via interactions with both CIR and GIRK1 subunits to gate the channel.

Cite This Study

Krapivinsky et al. (1995) studied this question.

synapsesocial.com/papers/6a73765161762fa209a729eahttps://doi.org/10.1074/jbc.270.49.29059
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Equilibrium calcium-calcium exchange in cardiac sarcolemmal vesicles.1983 · 158 citations
  2. 2Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers.1995 · 309 citations
  3. 3On the mechanism of G protein beta gamma subunit activation of the muscarinic K+ channel in guinea pig atrial cell membrane. Comparison with the ATP-sensitive K+ channel.1992 · 199 citations
  4. 4The α Subunit of the GTP Binding Protein G k Opens Atrial Potassium Channels1987 · 312 citations
  5. 5Atrial G protein-activated K+ channel: expression cloning and molecular properties.1993 · 359 citations