What question did this study set out to answer?

The aim is to explore how cAMP-dependent protein kinase affects heart contractile force under various conditions.

August 1, 1977AJP Heart and Circulatory Physiology

Involvement of cAMP-dependent protein kinase in the regulation of heart contractile force

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Population

Perfused working rat heart model

Design

Preclinical

Authors

SKS L KeelyKent State University JCJackie D. CorbinDartmouth College

Discussion

Member takes

Implication

Beta-adrenergic inotropy may act via cAMP-PKA separately from preload or calcium in rat hearts; leaves open translation to human therapies.

Key Points

The aim is to explore how cAMP-dependent protein kinase affects heart contractile force under various conditions.
Perfusion of rat hearts with epinephrine, a phosphodiesterase inhibitor, calcium, and altered filling pressure.
Evaluation of the relationship between cAMP levels, protein kinase activity, and contractile force.
Epinephrine led to rapid increases in cAMP and protein kinase activity prior to enhanced contractile force.
Epinephrine and the inhibitor synergistically boosted cAMP and contractile force.
Force increases from higher filling pressure or calcium did not alter cAMP or protein kinase activity.

Structured PICO

Population

Perfused working rat heart model

Intervention

Perfusate epinephrine, 1-methyl-3-isobutylxanthine, calcium, and increased filling pressure

Outcome

cAMP levels, protein kinase activity ratio, active phosphorylase, and contractile forcesurrogate

Beta-adrenergic stimulation increases myocardial contractile force via cAMP-dependent protein kinase activation, a mechanism distinct from the Frank-Starling response or calcium-induced inotropy.

Cite This Study

Keely et al. (1977) studied this question.

synapsesocial.com/papers/6a7395a91aa2fc9e78fb6ae6 https://doi.org/10.1152/ajpheart.1977.233.2.h269

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Implication

Beta-adrenergic inotropy may act via cAMP-PKA separately from preload or calcium in rat hearts; leaves open translation to human therapies.

Involvement of cAMP-dependent protein kinase in the regulation of heart contractile force

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Involvement of cAMP-dependent protein kinase in the regulation of heart contractile force

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider