November 28, 2006Journal of Biological ChemistryOpen Access

Functional Analysis of Individual Binding Activities of the Scaffold Protein eIF4G

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Population

Mammalian cells (in vitro and intact cells) and reticulocyte lysate

Design

Preclinical

Authors

THTracey M. HintonCSIRO Manufacturing MCMark J. ColdwellUniversity of Southampton GCGillian CarpenterUniversity of Sussex

Discussion

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Implication

Redundancy in translation initiation may permit selective modulation; leaves open validation in disease models before any therapeutic consideration.

Structured PICO

Population

Mammalian cells (in vitro and intact cells) and reticulocyte lysate

Intervention

eIF4GIf molecules lacking either the PABP-binding site, the eIF3-binding site, the middle domain eIF4A-binding site, or the C-terminal segment

Outcome

Ability to form complexes with eIF4E, enter 48 S complexes and polysomes, rescue translation, and support translation in reticulocyte lysatesurrogate

There is considerable redundancy in the mechanisms forming translation initiation complexes in mammalian cells, with many individual interactions having regulatory rather than essential roles.

Cite This Study

Hinton et al. (2006) studied this question.

synapsesocial.com/papers/6a73d9d68055c84dbef15f42 https://doi.org/10.1074/jbc.m602780200

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Implication

Redundancy in translation initiation may permit selective modulation; leaves open validation in disease models before any therapeutic consideration.

Functional Analysis of Individual Binding Activities of the Scaffold Protein eIF4G

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional Analysis of Individual Binding Activities of the Scaffold Protein eIF4G

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider