Translational activation of uncapped mRNAs by the central part of human eIF4G is 5′ end-dependent
Population
Cell-free translation reactions
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Remains preclinical; hypothesis-generating for eIF4G-mediated uncapped translation mechanisms.
Structured PICO
P
Population
Cell-free translation reactions
I
Intervention
Recombinant variants of human eIF4G lacking the N-terminal domain (aa 486-1404 and aa 486-935) expressed as GST-fusion proteins
O
Outcome
Effect on translation of capped and uncapped mRNAssurrogate
The central part of human eIF4G can activate translation of uncapped mRNAs in a 5' end-dependent manner without requiring the C-terminal eIF4A binding site.
Cite This Study
Gregorio et al. (1998) studied this question.