Key result
Cultivation of hERG-expressing cells at 30°C significantly increased functional cell surface hERG expression, yielding mean tail currents of 0.93 nA compared to 0.28 nA at 37°C.
Population
CHO-K1 and HEK293 cells stably expressing wild type hERG
Comparison
Cultivation at reduced temperatures for 3 to 7… vs Cultivation at standard temperature (37°C)
Design
Preclinical
Authors
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May refine hERG assay sensitivity for drug cardiac liability screening; leaves open clinical translation of improved currents.
Absolute Event Rate: 0.93% vs 0.28%
p-value: p=<0.0001
Cultivation of hERG-expressing cells at reduced temperatures (30°C) significantly boosts functional channel expression, improving the quality of high-throughput assays for detecting drug-induced cardiac liability.
Chen et al. (2007) studied In vitro hERG channel expression. Cultivation at reduced temperature (30°C) vs. Cultivation at 37°C was evaluated on hERG channel activity (mean tail current amplitude in nA) (p=<0.0001). Cultivation of hERG-expressing cells at 30°C significantly increased functional cell surface hERG expression, yielding mean tail currents of 0.93 nA compared to 0.28 nA at 37°C.
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