Key result
Increased temperature shifted the voltage dependence of hERG channel activation in the hyperpolarizing direction and inactivation in the depolarizing direction, increasing open state occupancy.
Why the study?
Does increased temperature alter the activation and inactivation kinetics of hERG channels expressed in Chinese hamster ovary cells?
Population
human ether-à-go-go related gene (hERG) K(+) channels expressed in Chinese hamster ovary cells
Comparison
Increased temperature (up to 37 degrees C) vs Room temperature
Design
Preclinical
Authors
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Temperature sensitivity precludes simple scaling of room-temperature hERG data; leaves open effects on in vivo repolarization or drug safety.
Does increased temperature alter the activation and inactivation kinetics of hERG channels expressed in Chinese hamster ovary cells?
The study demonstrates that hERG channel kinetics are highly temperature-sensitive, emphasizing that room temperature results cannot be simply extrapolated to physiological temperatures using a single scale factor.
Vandenberg et al. (2006) studied this question. Increased temperature vs. Room temperature was evaluated on Activation and inactivation processes of hERG channels. Increased temperature shifted the voltage dependence of hERG channel activation in the hyperpolarizing direction and inactivation in the depolarizing direction, increasing open state occupancy.
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