Key result
High-dose propofol significantly decreased heart rate to 324 bpm compared to 468 bpm with isoflurane, and blunted autonomic pharmacological responses, suggesting it is unsuitable for cardiovascular analysis in mice.
Why the study?
Does propofol alter electrocardiogram measures and autonomic responses compared to isoflurane or pentobarbital in mice?
Does propofol alter electrocardiogram measures and autonomic responses compared to isoflurane or pentobarbital in mice?
Absolute Event Rate: 324% vs 468%
p-value: p=<0.05
Propofol anesthesia significantly depresses heart rate and blunts autonomic responses in mice, indicating it is unsuitable for murine cardiovascular studies compared to isoflurane.
May warrant avoiding propofol in murine CV phenotyping; leaves open optimal anesthetic selection for autonomic studies.
We investigated the anesthetic effects of propofol on the electrocardiogram (ECG) in mice. We also compared the effects of isoflurane (2%) inhalation anesthesia, intraperitoneal propofol (50 or 100 mg/kg), and pentobarbital (50 mg/kg) on ECG in mice. Isoflurane inhalation and pentobarbital anesthesia were both associated with an acceptable heart rate (HR) range (ca. 450 - 500 bpm). In contrast, high-dose propofol anesthesia significantly decreased the HR. Importantly, propofol anesthesia led to significantly reduced responses to propranolol, a β-blocker, suggesting that it affects sympathetic tonus and is not suitable for the evaluation of cardiovascular or sympathetic function. Propofol also reduced the response to atropine, indicative of suppression of mouse parasympathetic nerve activity. Our data suggest that propofol anesthesia should not be the first choice for cardiovascular analysis in mice.
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Shintaku et al. (2014) studied General anesthesia for cardiovascular evaluation (n=74). Propofol vs. Isoflurane (2% inhalation) or Pentobarbital (50 mg/kg intraperitoneally) was evaluated on Heart rate (HR) (p=<0.05). High-dose propofol significantly decreased heart rate to 324 bpm compared to 468 bpm with isoflurane, and blunted autonomic pharmacological responses, suggesting it is unsuitable for cardiovascular analysis in mice.
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