In a rabbit cell-free translation system, the two codons downstream from the TMV UAG signal are important determinants of termination codon suppression.
Downstream codons dictate TMV UAG suppression in rabbit lysates; leaves open relevance to eukaryotic or in vivo translation.
To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstream from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo.
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Valle et al. (1992) studied this question.
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