What question did this study set out to answer?

To determine whether sodium pentobarbital exerts persistent depressant effects on isolated working rat heart performance and alters subsequent inotropic response to dobutamine.

March 1, 1986Journal of Cardiovascular Pharmacology

Sodium Pentobarbital Effects on Cardiac Function and Response to Dobutamine

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Why the study?

Does sodium pentobarbital exposure alter baseline cardiac function and subsequent response to dobutamine in isolated working rat hearts?

Population

Isolated working hearts from rats (killed after pentobarbital anesthesia or by cervical fracture)

Comparison

In vitro sodium pentobarbital treatment followed… vs Hearts from rats killed by cervical fracture and…

Design

Preclinical

Authors

LSLeigh D. SegelUniversity of California, Davis SRS. V. RendigUniversity of California, Davis

Discussion

Member takes

Implication

Pentobarbital induces transient depression in isolated rat hearts that largely reverses after washout; leaves open relevance to in vivo models or clinical anesthetic choice.

Key Points

To determine whether sodium pentobarbital exerts persistent depressant effects on isolated working rat heart performance and alters subsequent inotropic response to dobutamine.
Extracted isolated working hearts from rats euthanized either under pentobarbital anesthesia or by cervical fracture, comparing ventilated versus nonventilated conditions during excision.
Treated hearts with 0.4 mM sodium pentobarbital in vitro, followed by a washout period and subsequent administration of 5 x 10⁻⁸ M dobutamine.
Perfusion with 0.4 mM sodium pentobarbital significantly depressed hemodynamic and contractile function, with full recovery post-washout except for -dP/dtmax, which remained depressed by approximately 7%.
Prior pentobarbital exposure in vitro minimally altered the inotropic response to dobutamine, though hearts from cervical fracture euthanasia showed a smaller response than those from pentobarbital-anesthetized animals.
Hearts excised from ventilated animals exhibited significantly higher left ventricular peak systolic pressure, peak aortic flow rate, and peak power than hearts from nonventilated animals.

Structured PICO

Does sodium pentobarbital exposure alter baseline cardiac function and subsequent response to dobutamine in isolated working rat hearts?

Population

Isolated working hearts from rats (killed after pentobarbital anesthesia or by cervical fracture)

Intervention

In vitro sodium pentobarbital treatment (0.4 mM) followed by washout and subsequent dobutamine (5 X 10(-8) M) administration

Comparator

Hearts from rats killed by cervical fracture (no pentobarbital anesthesia) and nonventilated animals

Outcome

Hemodynamic and contractile functions (including -dP/dtmax and inotropic response to dobutamine)surrogate

Sodium pentobarbital has transient depressive effects on isolated rat heart function but minimally affects subsequent response to dobutamine after washout, while ventilation during excision improves isolated heart function.

Cite This Study

Segel et al. (1986) studied this question.

synapsesocial.com/papers/6a7481441646b2abe50327ed https://doi.org/10.1097/00005344-198603000-00024

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Implication

Pentobarbital induces transient depression in isolated rat hearts that largely reverses after washout; leaves open relevance to in vivo models or clinical anesthetic choice.

Sodium Pentobarbital Effects on Cardiac Function and Response to Dobutamine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Sodium Pentobarbital Effects on Cardiac Function and Response to Dobutamine

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider