Key result
Targeted ubiquitination of KCNQ1 and CaV1.2 channels using an engineered E3 ligase (nanoCHIP) eliminated their surface density and abolished reconstituted currents without affecting total protein expression.
Engineered E3 ligases can selectively target and ubiquitinate specific ion channels, enabling post-translational functional knockdown and mechanistic study of membrane protein trafficking.
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nanoCHIP enables selective channel removal in vitro; leaves open therapeutic translation to cardiac channelopathies.
Kanner et al. (2017) studied Ion channel regulation. Engineered E3 ubiquitin ligase (nanoCHIP) vs. Nanobody alone or catalytically inactive nanoCHIP was evaluated on Surface density and functional currents of targeted ion channels. Targeted ubiquitination of KCNQ1 and CaV1.2 channels using an engineered E3 ligase (nanoCHIP) eliminated their surface density and abolished reconstituted currents without affecting total protein expression.
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