November 29, 2001The FASEB JournalOpen Access

Creatine kinase is physically associated with the cardiac ATP‐sensitive k + channel in vivo

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Population

Guinea pig ventricular cardiomyocytes and A549 cells transfected with Kir6.2, SUR2A, and muscle creatine…

Design

Preclinical

Authors

RCRussell M. CrawfordFalmouth University HRHarri RankiUniversity of Helsinki CBCatherine H. BottingAndrews University

Discussion

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Implication

No immediate clinical implications; leaves open whether CK-SUR2A coupling modulates human cardiac excitability in ischemia.

Structured PICO

Population

Guinea pig ventricular cardiomyocytes and A549 cells (natively devoid of KATP channels) transfected with Kir6.2, SUR2A, and muscle creatine kinase.

Intervention

Application of creatine kinase substrates (creatine, phosphocreatine, ATP, ADP) during patch-clamp electrophysiology; immunoprecipitation and mass spectrometry.

Outcome

Physical association between creatine kinase and KATP channel subunits (SUR2A and Kir6.2) and functional regulation of channel activity.surrogate

Creatine kinase physically associates with the SUR2A subunit of cardiac KATP channels, providing a structural basis for the coupling of cellular metabolism and membrane excitability.

Cite This Study

Crawford et al. (2001) studied this question.

synapsesocial.com/papers/6a751d9b01b447f0dadc024b https://doi.org/10.1096/fj.01-0466fje

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Creatine kinase is physically associated with the cardiac ATP‐sensitive k + channel in vivo

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Creatine kinase is physically associated with the cardiac ATP‐sensitive k + channel in vivo

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider