January 14, 1986Biochemistry
Measurement of an individual rate constant in the presence of multiple exchanges: application to myocardial creatine kinase reaction
Population
Isovolumic rat hearts perfused under three different conditions
Comparison
Multiple saturation transfer method using 31P… vs Conventional saturation transfer (CST) method
Design
Preclinical
Authors
Discussion
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Implication
MST resolves CK flux discrepancies by accounting for ATP gamma-Pi exchange; challenges CST reliability in myocardial energetics research.
Structured PICO
P
Population
Isovolumic rat hearts perfused under three different conditions
I
Intervention
Multiple saturation transfer (MST) method using 31P nuclear magnetic resonance (NMR)
C
Comparator
Conventional saturation transfer (CST) method
O
Outcome
Forward and reverse fluxes of myocardial creatine kinase (CK)surrogate
The multiple saturation transfer method resolves the paradoxical discrepancy in myocardial creatine kinase flux measurements seen with conventional methods by accounting for ATP gamma in equilibrium Pi exchange.
Cite This Study
Uǧurbil et al. (1986) studied this question.