What question did this study set out to answer?

This investigation aims to demonstrate the generation of free radicals in the stunned myocardium of conscious dogs and to compare these findings with open-chest dogs.

August 1, 1993Journal of Clinical InvestigationOpen Access

Demonstration of free radical generation in the "stunned" myocardium in the conscious dog and identification of major differences between conscious and open-chest dogs.

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Population

Conscious and open-chest dogs undergoing a 15-min coronary occlusion

Comparison

Administration of alpha-phenyl N-tert-butyl… vs Open-chest dogs compared with conscious dogs

Design

Preclinical

Follow-up

3 hours after reperfusion

Authors

XLX Y LiBaylor College of Medicine PMPaul B. McCayOllscoil na Gaillimhe – University of Galway MZMarcel ZughaibProvidence Hospital

Discussion

Member takes

Implication

Open-chest models overestimate radical production in stunning; leaves open applicability of prior findings to conscious physiology.

Key Points

This investigation aims to demonstrate the generation of free radicals in the stunned myocardium of conscious dogs and to compare these findings with open-chest dogs.
Conscious dogs underwent a 15-min coronary occlusion.
Alpha-phenyl N-tert-butyl nitrone (PBN) was administered, and coronary venous plasma analyzed using electron paramagnetic resonance spectroscopy.
Computer simulation was employed to identify PBN adducts associated with radical generation.
PBN radical production peaked at 3 minutes post-reperfusion and continued for 1-3 hours.
Total release of PBN adducts was approximately five times greater in open-chest compared to conscious dogs.
A strong correlation (r = 0.77) was found between PBN adduct production and severity of contractile dysfunction.

Structured PICO

Population

Conscious and open-chest dogs undergoing a 15-min coronary occlusion

Intervention

Administration of alpha-phenyl N-tert-butyl nitrone (PBN) followed by reperfusion

Comparator

Open-chest dogs compared with conscious dogs

Outcome

Myocardial release of PBN radical adducts measured by electron paramagnetic resonance spectroscopysurrogate

Free radicals are generated in the stunned myocardium of conscious dogs, but the magnitude of production and subsequent contractile dysfunction are greatly exaggerated in open-chest models, suggesting previous open-chest studies may not accurately reflect conscious physiology.

Cite This Study

Li et al. (1993) studied this question.

synapsesocial.com/papers/6a754857b35e02fb150343ed https://doi.org/10.1172/jci116608

Topics

Women and heart disease

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Demonstration of free radical generation in the "stunned" myocardium in the conscious dog and identification of major differences between conscious and open-chest dogs.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Demonstration of free radical generation in the "stunned" myocardium in the conscious dog and identification of major differences between conscious and open-chest dogs.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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