The study demonstrates the feasibility of simultaneous recording of calcium signals and mechanical activity in vascular smooth muscle using fura 2, showing that calcium changes precede tension changes.
May guide integrated vascular smooth muscle assays; hypothesis-generating and should not yet influence clinical practice.
In rat aortic strips loaded with a Ca2+ indicator, fura 2, the Ca2+ signal and contractile tension were simultaneously recorded. On the addition of high K+ or noradrenaline, the 500 nm fluorescence produced by excitation at 340 nm increased and that produced by excitation at 380 nm decreased. EGTA decreased the 340 nm fluorescence and increased the 380 nm fluorescence in resting and stimulated muscles. Tension changes were always preceded by fluorescent changes.
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Ozaki et al. (1987) studied this question.
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