December 1, 1986FEBS Letters

Fura‐2 diffusion and its use as an indicator of transient free calcium changes in single striated muscle cells

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Population

Balanus nubilus myofibrillar bundles and voltage-clamped single muscle fibres

Design

Preclinical

Authors

MTM. TimmermanAmsterdam University Medical Centers CAChristopher AshleyAlbany Stratton VA Medical Center Albany

Discussion

Member takes

Implication

Fura-2 usable below ~6 μM in isolated striated muscle without immobilization; leaves open validation in intact cardiac preparations.

Structured PICO

Population

Balanus nubilus myofibrillar bundles and voltage-clamped single muscle fibres

Intervention

Fura-2 loading

Outcome

Fura-2 intracellular binding, apparent diffusion constants, and relationship between half-time for relaxation of force and fura-2 fluorescence transientssurrogate

Fura-2 can be used as an indicator of transient free calcium changes in single striated muscle cells without significant immobilization, though buffering occurs at concentrations above 6 microM.

Cite This Study

Timmerman et al. (1986) studied this question.

synapsesocial.com/papers/6a79374ba861e9f599320cfa https://doi.org/10.1016/0014-5793(86)81073-0

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