Mechanism of spontaneous excitability in human embryonic stem cell derived cardiomyocytes
Population
Mid-stage human embryonic stem cell-derived cardiomyocytes, including multicellular preparations in embryoid…
Comparison
Pharmacological blockade with TTX, nifedipine… vs Control conditions (unblocked).
Design
Preclinical
Authors
Discussion
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Implication
NaV1.5 current may drive automaticity in hESC-CMs; hypothesis-generating and leaves open validation in native human tissue.
Structured PICO
P
Population
Mid-stage human embryonic stem cell-derived cardiomyocytes (hES-CMs) (20-35 days post plating), including multicellular preparations in embryoid bodies and isolated cells (n=19 for patch-clamp).
I
Intervention
Pharmacological blockade with TTX (3-100 microm), nifedipine (1 microm), diltiazem (1 microm), or Cs+ (2 mm).
C
Comparator
Control conditions (unblocked).
O
Outcome
Conduction velocity, spontaneous electrical activity, and ionic currents (Na+, Ca2+, HCN, K+).surrogate
In mid-stage human embryonic stem cell-derived cardiomyocytes, the absence of background K+ current and presence of prominent NaV1.5 Na+ current creates conditions for spontaneous excitability.
Cite This Study
Satin et al. (2004) studied this question.