High-throughput screening assays successfully identified small molecule inhibitors of group I introns, which may be useful for understanding ribozyme catalysis or therapeutic intervention in group I intron-containing microorganisms.
Establishes scalable assays for group I intron inhibitors; leaves open therapeutic potential in microorganisms.
High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenable to high-throughput screening have been established. Small molecules that inhibit the functions of group I introns have been identified. These inhibitors should be useful in better understanding ribozyme catalysis or in therapeutic intervention of group I intron-containing microorganisms.
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Mei et al. (1996) studied this question.
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