High performance liquid chromatography was employed to rapidly separate tocopherols and their model compounds, chroman-6-ols. Good separation of these compounds was obtained. By use of naphthalene as an internal standard, tocopherol was exactly quantitated in the range from 0.3 to 3.0 μg. Tocopherols and a model compound dispersed in a cell culture medium were analysed with ease.
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Matsuo et al. (1977) studied this question.
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