Key result
Patients with chronic heart failure had a significantly higher proportion of CD25-positive T-cells in their pericardial fluid compared to non-heart failure patients (45.00% vs 24.92%, p<0.05).
Why the study?
Are inflammatory cytokines and T-cell activation markers increased in the pericardial fluid of patients with chronic heart failure compared to non-heart failure patients?
Observational (n=74)
No
Are inflammatory cytokines and T-cell activation markers increased in the pericardial fluid of patients with chronic heart failure compared to non-heart failure patients?
Absolute Event Rate: 45% vs 24.92%
p-value: p=<0.05
Chronic heart failure is associated with increased infiltration of inflammatory cells and enhanced expression of inflammatory cytokines in pericardial fluid, suggesting a role for systemic inflammation in CHF progression.
May indicate pericardial T-cell activation in chronic HF; hypothesis-generating and requires validation before clinical relevance.
Pericardial fluid, as a biochemical indicator of heart status, directly indicates pathological alteration to the heart. The accumulation of pericardial fluid can be attributed to an underlying systemic or local inflammatory process. However, the pericardial fluid expression of cellular surface markers, as well as several cytokines in chronic heart failure (CHF), remain unclear. In order to evaluate these issues further the pericardial fluid expression of several cytokines and the surface expression of activity markers between CHF patients and non-heart failure (NHF) patients were analyzed. The pericardial fluid expression of cytokines was measured by immunofluorescence and biomarker of plasma N-terminal propeptide of B-type natriuretic peptide (NT-proBNP), while pericardial fluid levels of soluble glycoprotein 130 (sgp130) were analyzed by ELISA in 50 CHF and 24 NHF patients. In addition, the surface expression of activation markers for T-cells was measured by immunohistochemistry. Patients with CHF demonstrated increased levels of plasma NT-proBNP and pericardial fluid sgp130. Surface expression of cellular activation markers CD25 and Foxp3 in the pericardial fluid was increased in patients with CHF. Moreover, the pro- and anti-inflammatory cytokines interferon (IFN)-γ, interleukin (IL)-6 and IL-10 in patients with CHF also demonstrated an increased expression within its pericardial fluid. In addition, there was infiltration of inflammatory cells and enhanced expression of inflammatory cytokines in the pericardial fluid of patients with CHF, which may reflect T cell activation, suggesting that systemic inflammation is important in the progression of CHF. This evidence could indicate a possible novel target for future therapeutics and prevention of CHF.
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Iskandar et al. (2017) conducted an observational in Chronic heart failure (n=74). Chronic heart failure vs. Non-heart failure was evaluated on Proportion of CD25-positive cells in pericardial fluid (p=<0.05). Patients with chronic heart failure had a significantly higher proportion of CD25-positive T-cells in their pericardial fluid compared to non-heart failure patients (45.00% vs 24.92%, p<0.05).
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