Key result
Curcumin inhibited the osteogenic differentiation of human aortic valve interstitial cells by interfering with the activation of NF-κB, AKT, and ERK signaling pathways.
Why the study?
Osteogenic differentiation of human aortic valve interstitial cells is the key cellular mechanism of calcified aortic valve disease, motivating exploration into how curcumin inhibits this differentiation and its underlying molecular mechanisms.
Does curcumin inhibit osteogenic differentiation in human aortic valve interstitial cells?
Does curcumin inhibit osteogenic differentiation in human aortic valve interstitial cells?
Curcumin inhibits osteogenic differentiation of human aortic valve interstitial cells by interfering with NF-κB, AKT, and ERK pathways, suggesting a potential therapeutic role in calcified aortic valve disease.
Hypothesis-generating for curcumin in calcific aortic valve disease; requires clinical validation before therapeutic consideration.
The osteogenic differentiation of human aortic valve interstitial cells (hVICs) is the key cellular mechanism of calcified aortic valve disease (CAVD). This study aimed to explore how curcumin (CCM) inhibits the osteogenic differentiation of hVICs and elucidate the molecular mechanisms involved. In this study, CCM inhibited the osteogenic differentiation of hVICs under osteogenic medium (OM) conditions by reversing the OM-induced increase in calcified nodule formation and osteogenesis-specific markers (ALP and Runx2). RNA sequencing identified 475 common differentially expressed genes with Venn diagrams of the different groups. Kyoto Encyclopedia of Genes and Genomes enrichment revealed that the CCM inhibition of hVIC osteogenic differentiation was enriched in the NF-κB, PI3K-AKT, TNF, Jak-STAT, and MAPK signaling pathways. In addition, CCM suppressed the phosphorylation of ERK, IκBα, AKT, and interfered with the translocation of P65 into the cell nucleus in hVICs under OM culture conditions. In conclusion, CCM inhibited the osteogenic differentiation of hVICs via interfering with the activation of NF-κB/AKT/ERK signaling pathways. Our findings provide novel insights into a critical role for CCM in CAVD progression and shed new light on CCM-directed therapeutics for CAVD.
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Zhou et al. (2020) studied Calcified aortic valve disease. Curcumin vs. Osteogenic medium alone was evaluated on Osteogenic differentiation of human aortic valve interstitial cells (calcified nodule formation and osteogenesis-specific markers). Curcumin inhibited the osteogenic differentiation of human aortic valve interstitial cells by interfering with the activation of NF-κB, AKT, and ERK signaling pathways.
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