What question did this study set out to answer?

To evaluate the diagnostic utility of detecting neutralizing immunoglobulin M (IgM) antibodies in acute-phase serum compared to traditional virus isolation for the rapid diagnosis of enterovirus infections.

April 1, 1989Journal of Clinical MicrobiologyOpen Access

Comparative evaluation of immunoglobulin M neutralizing antibody response in acute-phase sera and virus isolation for the routine diagnosis of enterovirus infection

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Why the study?

Does IgM neutralizing antibody response in acute-phase serum improve the rapid diagnosis of enterovirus infection compared to virus isolation in patients with viral symptoms?

Key Points

To evaluate the diagnostic utility of detecting neutralizing immunoglobulin M (IgM) antibodies in acute-phase serum compared to traditional virus isolation for the rapid diagnosis of enterovirus infections.
Evaluated N=314 hospitalized patients presenting with acute symptoms suggestive of viral infection.
Obtained acute-phase serum alongside peripheral samples (throat swabs, stool, or both) during early hospitalization.
Fractionated serum IgM using ion-exchange chromatography and tested for neutralizing activity against circulating enterovirus serotypes in comparison with cell culture virus isolation.
Concordant results occurred in 67% of cases, with 189 patients (60%) negative and 21 patients (7%) positive by both diagnostic methods.
Discordant results occurred in 104 patients: 51 (16%) were positive solely by virus isolation, and 53 (17%) were positive solely by IgM serology.
Among the 21 patients positive by both methods, matching serotypes were identified in 9 cases (43%), while 12 cases (57%) showed different serotypes due to cross-reactivity.

Structured PICO

Does IgM neutralizing antibody response in acute-phase serum improve the rapid diagnosis of enterovirus infection compared to virus isolation in patients with viral symptoms?

Population

314 patients exhibiting symptoms consistent with a viral disease

Intervention

Neutralizing immunoglobulin M (IgM) antibody response evaluation in acute-phase serum

Comparator

Virus isolation from peripheral site (throat or stools or both)

Outcome

Detection of enterovirus infectionsurrogate

IgM neutralizing antibody response on acute-phase serum is of limited value for the rapid diagnosis of acute enterovirus infection compared to virus isolation.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Acute-phase IgM shows limited concordance with isolation; leaves open its added value for rapid enterovirus diagnosis pending validation.

Cite This Study

Pozzetto et al. (1989) studied this question.

synapsesocial.com/papers/6a75b7dbae41e4ffbd6a3c98 https://doi.org/10.1128/jcm.27.4.705-708.1989

View Full Paper

Ask AI

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Comparative evaluation of immunoglobulin M neutralizing antibody response in acute-phase sera and virus isolation for the routine diagnosis of enterovirus infection

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Comparative evaluation of immunoglobulin M neutralizing antibody response in acute-phase sera and virus isolation for the routine diagnosis of enterovirus infection

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider