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A mixture of buffalo casein and glucose of high moisture content was given different heat treatments which greatly reduced the value of the materials as a protein source for young rats. Their value as a source of lysine fell much more than their value as a source of methionine or ‘methionine + cystine’. These feeding results confirmed the results of two laboratory prediction tests—reactivity with fluorodinitrobenzene for ‘available’ lysine, and a microbiological assay for ‘available’ methionine with Strep. zymogenes NCDO 592 on mild papain digests.
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Rao et al. (1963) studied this question.
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