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The development of a multiplex PCR method enables rapid screening for recombinant vaccine-derived polioviruses, supporting global poliovirus surveillance.
The recent discovery of recombinant circulating vaccine-derived poliovirus (recombinant cVDPV) has highlighted the need for enhanced global poliovirus surveillance to assure timely detection of any future cVDPV outbreaks. Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR.
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Kilpatrick et al. (2004) studied this question.
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