Why the study?
Does PCR-based gene amplification improve the detection of viral persistence in the hearts of mice with experimental coxsackievirus B3 myocarditis compared to viral isolation?
Population
Weanling C3H/He mice with experimental coxsackievirus B3 (Nancy strain) myocarditis
Comparison
Polymerase chain reaction after reverse… vs Viral isolation and slot-blot hybridization
Design
Preclinical
Follow-up
up to 180 days
Authors
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PCR extends detection of viral persistence in murine myocarditis models; leaves open relevance to human diagnosis or therapy.
Does PCR-based gene amplification improve the detection of viral persistence in the hearts of mice with experimental coxsackievirus B3 myocarditis compared to viral isolation?
PCR-based gene amplification allows for the detection of persistent viral RNA in experimental myocarditis even after infectious virus is no longer detectable by standard isolation methods.
Koide et al. (1992) studied this question.
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