Myocardium miRNA Analysis Reveals Potential Biomarkers of Sudden Coronary Death in Rats

Figures

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  Figure 1. Pathological and electrocardiogram changes after the establishment of the rat model. (A–D). Pathological changes in coronary arteries in ASD, AS, AMI, and sham groups (200×). (E–H). Pathological changes in cardiomyocytes in ASD, AS, AMI, and sham groups (200×). (I). The electrocardiogram of the ASD group. (J). The electrocardiogram of the AMI group. (K). The electrocardiogram of the sham group. (L). Cardiac ischemia map after coronary artery ligation in rats. (M,N). Statistical difference chart of ST amplitude and bpm of ASD, AMI, and sham group. ASD: atherosclerotic death, AS: coronary atherosclerosis, AMI: acute myocardial ischemia. Data are pooled from two independent experiments. Data are shown as means ± SEMS; *** p < 0.001.

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  Figure 2. Differential expression of miRNA in rat myocardium. (A). Volcano plot of differentially expressed miRNAs in ASD group; (B). Volcano plot of differentially expressed miRNAs in AS group; (C). Volcano plot of differentially expressed miRNAs in AMI group. Up-regulated miRNA expression is shown in red, and down-regulated miRNA expression is shown in green. (D). Heat map of differentially expressed miRNAs in ASD group; (E). Heat map of differentially expressed miRNAs in AS group; (F). Heat map of differentially expressed miRNAs in AMI group. Up-regulated miRNA expression is shown in red and down-regulated miRNA expression is shown in blue. Sample 1–6: ASD group; Sample 7–12: AS group; Sample 13–18: AMI group; Sample 19–24: Sham group.

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  Figure 3. Target gene analysis of differential miRNA. (A). The KEGG analysis in the ASD group; (B). the KEGG analysis in the AS group; and (C). the KEGG analysis in the AMI group. The horizontal axis represents the enrichment factor, the vertical axis shows the pathways, and the size of the circles indicates the number of enriched genes, while the color of the circles represents the q-value.

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  Figure 4. miRNA-mRNA regulatory network and identification of key miRNAs. (A). miRNA-mRNA regulatory network of the ASD group; (B). miRNA-mRNA regulatory network of the AMI group; (C). the top 10 key genes in the ASD group; (D). Venn diagram of differential miRNAs in the ASD group and AS and AMI group; (E). LASSO egression plot of the ASD group; (F). LASSO egression plot of the AMI group; (G). ROC curve graph of the ASD group; (H). ROC curve graph of the AMI group.