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January 15, 1991Biochemical JournalOpen Access

Antibodies as probes for ligand gating of single sarcoplasmic reticulum Ca2+-release channels

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Population

Junctional sarcoplasmic reticulum (SR) vesicles fused into planar lipid bilayers

Design

Preclinical

Authors

MFMichael FillElectrophysiologyRMRafael Mejía-AlvarezMidwestern UniversityFZFrancesco ZorzatoElectrophysiology

Discussion

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Implication

Supports targeted anti-RYR antibodies as in vitro probes for SR Ca2+ channel gating; leaves open relevance to cardiac disease models or therapy.

Structured PICO

P
Population
Junctional sarcoplasmic reticulum (SR) vesicles fused into planar lipid bilayers
I
Intervention
Polyclonal antibodies against the SR junctional face membrane (anti-JFM) and the ryanodine receptor (anti-RYR)
O
Outcome
Single-channel gating properties (open probability and conductance) of the RYR SR Ca2+-release channelsurrogate

Antibodies directed at specific regions of the ryanodine receptor can be used as effective probes to study the structural and functional properties of SR Ca2+-release channel gating at the single-channel level.

Cite This Study

Fill et al. (1991) studied this question.

synapsesocial.com/papers/6a764dfa8e31ea4274087f62https://doi.org/10.1042/bj2730449
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Role of Malignant Hyperthermia Domain in the Regulation of Ca2+ Release Channel (Ryanodine Receptor) of Skeletal Muscle Sarcoplasmic Reticulum1996 · 37 citations
  2. 2Identification of the domain recognized by anti-(ryanodine receptor) antibodies which affect Ca2+-induced Ca2+ release1993 · 30 citations
  3. 3Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel.1987 · 234 citations
  4. 4Ryanodine Receptor Oligomeric Interaction2004 · 14 citations
  5. 5Insights into channel modulation mechanism of RYR1 mutants using Ca2+ imaging and molecular dynamics2019 · 15 citations