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May 1, 1993Biochemical JournalOpen Access

Identification of the domain recognized by anti-(ryanodine receptor) antibodies which affect Ca2+-induced Ca2+ release

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Population

Rabbit skeletal muscle ryanodine receptor cDNA fragments expressed in E. coli and isolated terminal…

Comparison

Anti- antibodies vs Preimmune IgG

Design

Preclinical

Authors

STSusan TrevesUniversity of FerraraPCPaola ChiozziUniversity of FerraraFZFrancesco ZorzatoElectrophysiology

Discussion

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Implication

Hypothesis-generating for Ca2+-activating domain localization near loop 2; leaves open cardiac translation and should not change practice.

Structured PICO

P
Population
Rabbit skeletal muscle ryanodine receptor cDNA fragments expressed in E. coli (JM 101 strain) and isolated terminal cisternae from sarcoplasmic reticulum
I
Intervention
Anti-(ryanodine receptor) antibodies (PC8-affinity-purified and anti-PC15 fusion protein antibodies)
C
Comparator
Preimmune IgG
O
Outcome
Doxorubicin-induced Ca2+ release and Ca2+-induced 45Ca2+ efflux from isolated terminal cisternaesurrogate

The Ca2+-activating domain of the skeletal muscle Ca2+-release channel is close to or associated with myoplasmic loop 2 (residues 4380-4625).

Cite This Study

Treves et al. (1993) studied this question.

synapsesocial.com/papers/6a8c1d451692ae2bc0f47e0dhttps://doi.org/10.1042/bj2910757
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Antibodies as probes for ligand gating of single sarcoplasmic reticulum Ca2+-release channels1991 · 28 citations
  2. 2Electron probe X-ray microanalysis of post-tetanic Ca2+ and Mg2+ movements across the sarcoplasmic reticulum in situ.1985 · 111 citations
  3. 3Molecular cloning of cDNA encoding the Ca2+ release channel (ryanodine receptor) of rabbit cardiac muscle sarcoplasmic reticulum.1990 · 630 citations
  4. 4PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT1951 · 318,728 citations
  5. 5Prediction of protein antigenic determinants from amino acid sequences.1981 · 3,747 citations