January 1, 1998Journal of Biological ChemistryOpen Access

Structural Determinants of Ligand and Cell Surface Binding of Insulin-like Growth Factor-binding Protein-3

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Population

Chinese hamster ovary cells expressing five recombinant human IGFBP-3 variants

Comparison

Five recombinant human IGFBP-3 variants vs rhIGFBP-3 (wild-type)

Design

Preclinical

Authors

SFSue M. FirthThe University of Sydney UGUsha GaneshprasadRoyal North Shore Hospital RBRobert C. BaxterThe University of Sydney

Discussion

Member takes

Implication

Highlights IGFBP-3 residues 228-232 as essential for ALS binding; leaves open in vivo effects on IGF regulation.

Structured PICO

Population

Chinese hamster ovary cells expressing five recombinant human IGFBP-3 variants

Intervention

Five recombinant human IGFBP-3 variants (deletions of amino acids 89-264, 89-184, and 185-264, and site-specific mutations 228KGRKR --> MDGEA and 253KED --> RGD)

Comparator

rhIGFBP-3 (wild-type)

Outcome

Ligand binding (IGF affinity), ALS binding affinity, and cell surface associationsurrogate

Residues 228-232 of IGFBP-3 are essential for cell association and normal ALS binding affinity.

Cite This Study

Firth et al. (1998) studied this question.

synapsesocial.com/papers/6a767e3107527c8c91c44732 https://doi.org/10.1074/jbc.273.5.2631

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Structural Determinants of Ligand and Cell Surface Binding of Insulin-like Growth Factor-binding Protein-3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Structural Determinants of Ligand and Cell Surface Binding of Insulin-like Growth Factor-binding Protein-3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider