November 1, 1988Journal of Biological ChemistryOpen Access

Trypsin destruction of the high affinity ryanodine binding sites of the junctional sarcoplasmic reticulum.

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Population

Junctional sarcoplasmic reticulum membranes

Comparison

Tryptic digestion in sucrose buffer vs Tryptic digestion in NaCl buffer or sucrose…

Design

Preclinical

Authors

VSVarda Shoshan‐BarmatzBen-Gurion University of the Negev AZAliza ZarkaBen-Gurion University of the Negev

Discussion

Member takes

Implication

Suggests RyR fragment importance in SR; leaves open relevance to human cardiac disease and therapy.

Structured PICO

Population

Junctional sarcoplasmic reticulum membranes

Intervention

Tryptic digestion in sucrose buffer

Comparator

Tryptic digestion in NaCl buffer or sucrose buffer with MgCl2

Outcome

Ryanodine binding capacity and cleavage of the 135-kDa tryptic fragmentsurrogate

This basic science study demonstrates that the 135-kDa tryptic fragment of the ryanodine receptor is essential for maintaining ryanodine binding capacity in the junctional sarcoplasmic reticulum.

Cite This Study

Shoshan‐Barmatz et al. (1988) studied this question.

synapsesocial.com/papers/6a7699b22e130d02afec12ec https://doi.org/10.1016/s0021-9258(18)37458-1

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Trypsin destruction of the high affinity ryanodine binding sites of the junctional sarcoplasmic reticulum.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Trypsin destruction of the high affinity ryanodine binding sites of the junctional sarcoplasmic reticulum.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider