What question did this study set out to answer?

To examine the presence and electrophysiological properties of ouabain-induced oscillatory afterpotentials in fresh and organ-cultured young embryonic chick hearts.

October 1, 1984Circulation ResearchOpen Access

Properties of oscillatory afterpotentials in young embryonic chick hearts.

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Population

Young (3-day-old) embryonic chick hearts, both fresh and organ cultured for 6-14 days

Design

Preclinical

Authors

MKMichio KojimaJikei University School of Medicine NSN. SperelakisUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

Embryonic chick model supports early channel ontogeny; leaves open translation to human developmental arrhythmias.

Key Points

To examine the presence and electrophysiological properties of ouabain-induced oscillatory afterpotentials in fresh and organ-cultured young embryonic chick hearts.
Examined 3-day-old embryonic chick hearts, testing both fresh preparations and those organ-cultured for 6-14 days.
Suppressed automaticity by raising extracellular potassium from 4 mM to 6 mM and electrically paced preparations from 0.5 Hz up to 4 Hz.
Induced oscillatory afterpotentials with 1.3-7.5 µM ouabain while modifying extracellular concentrations of sodium, calcium, barium, strontium, magnesium, and manganese.
Stable oscillatory afterpotentials were induced by 1.3-7.5 µM ouabain, and their amplitudes increased when stimulation rates rose from 0.5 Hz to 3 and 4 Hz.
Oscillatory afterpotential amplitudes were potentiated by elevating extracellular calcium to 3.6 mM or adding 0.1 mM barium.
Oscillatory afterpotentials were significantly depressed by low extracellular sodium (40-121 mM), 4 mM strontium, 2-4 mM magnesium, and 1-4 mM manganese.

Structured PICO

Population

Young (3-day-old) embryonic chick hearts, both fresh and organ cultured for 6-14 days

Intervention

Ouabain (1.3-7.5 microM) with electrical stimulation (0.5-4 Hz) and varying extracellular ion concentrations (K+, Ca++, Ba++, Na+, Sr++, Mg++, Mn++)

Outcome

Presence and properties (amplitude, potentiation, depression) of ouabain-induced oscillatory afterpotentialssurrogate

Young embryonic chick hearts exhibit ouabain-induced oscillatory afterpotentials similar to adult mammalian tissues, suggesting the early developmental presence of calcium-operated mixed cation channels.

Cite This Study

Kojima et al. (1984) studied this question.

synapsesocial.com/papers/6a76b82ede5e3adfbdf6c616 https://doi.org/10.1161/01.res.55.4.497

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Properties of oscillatory afterpotentials in young embryonic chick hearts.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Properties of oscillatory afterpotentials in young embryonic chick hearts.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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